Figure 3.
Mutational analysis of NBR1 reveals unique binding determinants for MAP1LC3A, GABARPL1, FIP200Claw, and TAX1BP1CC2. (A–D) Mutational peptide array of NBR1728–747 probed with HA-LC3A (A), MBP-GABARAPL1 (B), GST-FIP200Claw (C), or TAX1BP1CC2-MBP (D), and immunoblotted with HRP-conjugated antibodies to GST or MBP. Each amino acid position is substituted for every other amino acid. NBR1’s canonical LIR motif (YIII) is indicated in blue. Red arrows indicate key residues identified for each SLiM binding motif. Membrane was cut where indicated to facilitate stripping. Refer to the image caption for details.

Mutational analysis of NBR1 reveals unique binding determinants for MAP1LC3A, GABARPL1, FIP200 Claw , and TAX1BP1 CC2 . (A–D) Mutational peptide array of NBR1728–747 probed with HA-LC3A (A), MBP-GABARAPL1 (B), GST-FIP200Claw (C), or TAX1BP1CC2-MBP (D), and immunoblotted with HRP-conjugated antibodies to GST or MBP. Each amino acid position is substituted for every other amino acid. NBR1’s canonical LIR motif (YIII) is indicated in blue. Red arrows indicate key residues identified for each SLiM binding motif. Membrane was cut where indicated to facilitate stripping.

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