GRIK1 localization in WT, FAT3, and PTPσ mutant retinas. (A) Immunostaining for GRIK1 (magenta) in the OPL region of WT retinal sections. (B) Immunostaining for GRIK1 (magenta) in Fat3∆TM/∆TM retinal sections. (A′ and B′) Cone arrestin (ARR3) (white) labels the cone photoreceptor axonal endings in the OPL in A′ and B′. (C) Quantification of GRIK1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.06 (n = 13 animals, 60 retinal regions); Fat3∆TM/∆TM: 0.56 ± 0.04 (n = 12 animals, 54 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. (D) Immunostaining for GRIK1 (magenta) in the OPL region of WT retinal sections. (E) Immunostaining for GRIK1 (magenta) in the OPL region of Fat3∆ICD-GFP/∆ICD-GFP retinal sections. (D′ and E′) Cone arrestin (ARR3) (white) labels the cone photoreceptor endings in the OPL in D′ and E′. (F) Quantification of GRIK1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.04 (n = 8 animals, 32 retinal regions); Fat3∆ICD/∆ICD: 0.75 ± 0.04 (n = 8 animals, 32 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. (G) Immunostaining for GRIK1 (magenta) in the OPL region of WT retinal sections. (H) Immunostaining for GRIK1 (magenta) in Ptprs−/− retinal sections. (G′ and H′) Cone arrestin (ARR3) (white) labels the cone photoreceptor endings in the OPL in G′ and H′. (I) Quantification of GRIK1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.06 (n = 6 animals, 30 retinal regions); Ptprs−/−: 0.66 ± 0.04 (n = 6 animals, 30 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. Scale bars: 20 µm.
Figure 7.

GRIK1 localization in WT, FAT3, and PTPσ mutant retinas. (A) Immunostaining for GRIK1 (magenta) in the OPL region of WT retinal sections. (B) Immunostaining for GRIK1 (magenta) in Fat3∆TM/∆TM retinal sections. (A′ and B′) Cone arrestin (ARR3) (white) labels the cone photoreceptor axonal endings in the OPL in A′ and B′. (C) Quantification of GRIK1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.06 (n = 13 animals, 60 retinal regions); Fat3∆TM/∆TM: 0.56 ± 0.04 (n = 12 animals, 54 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. (D) Immunostaining for GRIK1 (magenta) in the OPL region of WT retinal sections. (E) Immunostaining for GRIK1 (magenta) in the OPL region of Fat3∆ICD-GFP/∆ICD-GFP retinal sections. (D′ and E′) Cone arrestin (ARR3) (white) labels the cone photoreceptor endings in the OPL in D′ and E′. (F) Quantification of GRIK1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.04 (n = 8 animals, 32 retinal regions); Fat3∆ICD/∆ICD: 0.75 ± 0.04 (n = 8 animals, 32 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. (G) Immunostaining for GRIK1 (magenta) in the OPL region of WT retinal sections. (H) Immunostaining for GRIK1 (magenta) in Ptprs−/− retinal sections. (G′ and H′) Cone arrestin (ARR3) (white) labels the cone photoreceptor endings in the OPL in G′ and H′. (I) Quantification of GRIK1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.06 (n = 6 animals, 30 retinal regions); Ptprs−/−: 0.66 ± 0.04 (n = 6 animals, 30 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. Scale bars: 20 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal