Expression pattern of postsynaptic components PTPσ, HOMER1, GRIK1, and GRM6 in the retina (Related toFigs. 6 and 7). (A and B) PTPσ immunostaining in WT retinas B. PTPσ immunostaining in Ptprs−/− retinas. (A′ and B′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in A′ and B′. (C) Schematic representation of OFF-BCs and their synapses with cone photoreceptors. ARR3 (white) labels cones and GRIK1 (magenta) labels postsynaptic BC dendrites. (D) Schematic representation of ON-BCs and their synapses with cone photoreceptors. ARR3 (white) labels cones and GRM6 (magenta) labels postsynaptic cone and rod BC (RBC) dendrites. (E and E′) GRIK1 and GRM6 (E′) immunostaining of adult WT retina. (F and F′) GRIK1 and GRM6 (F′) immunostaining of Grik1−/− retina. (G and G′) GRIK1 and GRM6 (G′) immunostaining of Grm6−/− retina. (H) Immunostaining for CtBP2, a marker of presynaptic ribbon in WT retinas. (I) Immunostaining for CtBP2 Fat3∆TM/∆TM retinas. (H′ and I′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in H′ and I′. (J) Quantification of CtBP2 mean fluorescence intensity in the OPL, normalized by ONL signal. WT controls: 1.00 ± 0.02 (n = 8 animals, 36 retinal regions); Fat3∆TM/∆TM: 1.11 ± 0.02 (n = 8 animals, 36 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (K) Immunostaining for CtBP2 in WT retinas, littermates of Fat3∆ICD-GFP/∆ICD-GFP animals. (L) Immunostaining for CtBP2 in Fat3∆ICD-GFP/∆ICD-GFP retinas. (K′ and L′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in K′ and L′. (M) Quantification of CtBP2 mean fluorescence intensity in the OPL, normalized by ONL signal. WT controls: 1.00 ± 0.02 (n = 9 animals, from 36 retinal regions); Fat3∆ICD/∆ICD: 1.03 ± 0.02 (n = 9 animals, from 36 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. Scale bars: 20 µm. Error bars: SEM.
Figure S5.

Expression pattern of postsynaptic components PTPσ, HOMER1, GRIK1, and GRM6 in the retina (Related to Figs. 6 and 7). (A and B) PTPσ immunostaining in WT retinas B. PTPσ immunostaining in Ptprs−/− retinas. (A′ and B′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in A′ and B′. (C) Schematic representation of OFF-BCs and their synapses with cone photoreceptors. ARR3 (white) labels cones and GRIK1 (magenta) labels postsynaptic BC dendrites. (D) Schematic representation of ON-BCs and their synapses with cone photoreceptors. ARR3 (white) labels cones and GRM6 (magenta) labels postsynaptic cone and rod BC (RBC) dendrites. (E and E′) GRIK1 and GRM6 (E′) immunostaining of adult WT retina. (F and F′) GRIK1 and GRM6 (F′) immunostaining of Grik1−/− retina. (G and G′) GRIK1 and GRM6 (G′) immunostaining of Grm6−/− retina. (H) Immunostaining for CtBP2, a marker of presynaptic ribbon in WT retinas. (I) Immunostaining for CtBP2 Fat3∆TM/∆TM retinas. (H′ and I′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in H′ and I′. (J) Quantification of CtBP2 mean fluorescence intensity in the OPL, normalized by ONL signal. WT controls: 1.00 ± 0.02 (n = 8 animals, 36 retinal regions); Fat3∆TM/∆TM: 1.11 ± 0.02 (n = 8 animals, 36 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (K) Immunostaining for CtBP2 in WT retinas, littermates of Fat3∆ICD-GFP/∆ICD-GFP animals. (L) Immunostaining for CtBP2 in Fat3∆ICD-GFP/∆ICD-GFP retinas. (K′ and L′) Cone arrestin (ARR3) labels the cone photoreceptors endings in the OPL in K′ and L′. (M) Quantification of CtBP2 mean fluorescence intensity in the OPL, normalized by ONL signal. WT controls: 1.00 ± 0.02 (n = 9 animals, from 36 retinal regions); Fat3∆ICD/∆ICD: 1.03 ± 0.02 (n = 9 animals, from 36 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. Scale bars: 20 µm. Error bars: SEM.

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