PTPσ and HOMER1 localization in WT and FAT3 mutant retinas. (A) Western blot for PTPσ in protein lysate (input), in the supernatant after pulldown with a GST fusion to FAT-ICD (sup), and in the pellet after pulldown with GST alone (GST) or a FAT3-ICD GST fusion protein. (B and B′) Immunostaining of PTPσ (yellow) and GRIK1 (magenta, B′) in the OPL region of WT retinal sections. (C and C″) Immunostaining of PTPσ (yellow) and CtBP2 (cyan, C″), a marker of presynaptic terminals in photoreceptor axons. (D and D′) Immunostaining of PTPσ (yellow) and ARR3 (white) and PTPσ (D′) alone in the OPL region of WT retinal sections. (E and E′) Immunostaining of PTPσ (yellow) and ARR3 (white) and PTPσ (E′) alone in the OPL region of Fat3∆TM/∆TM retinal sections. (F) Quantification of PTPσ integrated intensity (normalized) in the OPL. WT Controls: 1.00 ± 0.07 (n = 4 animals, 16 retinal regions); Fat3∆TM/∆TM: 0.56 ± 0.05 (n = 3 animals, 12 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. (G and G′) Immunostaining of HOMER1 (yellow) and SV2 (cyan), and HOMER1 (G′) alone in WT retinal sections. (H and H′) Immunostaining of HOMER1 (yellow) and SV2 (cyan), and HOMER1 (H′) alone in Fat3∆TM/∆TM retinal sections. (I) Quantification of HOMER1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.07 (n = 3 animals, eight retinal regions); Fat3∆TM/∆TM: 0.91 ± 0.06 (n = 3 animals, eight retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. Scale bars: 20 µm. Source data are available for this figure: SourceData F6.
Figure 6.

PTPσ and HOMER1 localization in WT and FAT3 mutant retinas. (A) Western blot for PTPσ in protein lysate (input), in the supernatant after pulldown with a GST fusion to FAT-ICD (sup), and in the pellet after pulldown with GST alone (GST) or a FAT3-ICD GST fusion protein. (B and B′) Immunostaining of PTPσ (yellow) and GRIK1 (magenta, B′) in the OPL region of WT retinal sections. (C and C″) Immunostaining of PTPσ (yellow) and CtBP2 (cyan, C″), a marker of presynaptic terminals in photoreceptor axons. (D and D′) Immunostaining of PTPσ (yellow) and ARR3 (white) and PTPσ (D′) alone in the OPL region of WT retinal sections. (E and E′) Immunostaining of PTPσ (yellow) and ARR3 (white) and PTPσ (E′) alone in the OPL region of Fat3∆TM/∆TM retinal sections. (F) Quantification of PTPσ integrated intensity (normalized) in the OPL. WT Controls: 1.00 ± 0.07 (n = 4 animals, 16 retinal regions); Fat3∆TM/∆TM: 0.56 ± 0.05 (n = 3 animals, 12 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. (G and G′) Immunostaining of HOMER1 (yellow) and SV2 (cyan), and HOMER1 (G′) alone in WT retinal sections. (H and H′) Immunostaining of HOMER1 (yellow) and SV2 (cyan), and HOMER1 (H′) alone in Fat3∆TM/∆TM retinal sections. (I) Quantification of HOMER1 integrated intensity in the OPL. WT Controls: 1.00 ± 0.07 (n = 3 animals, eight retinal regions); Fat3∆TM/∆TM: 0.91 ± 0.06 (n = 3 animals, eight retinal regions). Each data point corresponds to a retinal region, color-coded by animal, nested two-tailed test. Error bar: SEM. Scale bars: 20 µm. Source data are available for this figure: SourceData F6.

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