BC and cones numbers, visualization of Grik1+ BCs morphology via injection of an AVV-Grik1-GFP virus in Fat3 mutants (Related toFigs. 3 and 4). (A) ARR3 immunohistochemistry of WT retinas. (A′) shows ARR3 staining together with DAPI staining. (B) ARR3 immunohistochemistry of Fat3∆TM/∆TM retinas. (B′) shows ARR3 staining together with DAPI staining. (C) VSX2 immunostaining of WT retinas. (D) VSX2 immunostaining of Fat3∆TM/∆TM retinas. (E) Quantification of number of cones marked by ARR3, as shown in A and B. WT controls: 27.77 ± 0.907 (n = 4 animals, 13 retinal regions); Fat3∆TM/∆TM: 29.00 ± 0.593 (n = 4 animals, 14 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (F) Quantification of thickness of area occupied by VSX2 staining, as shown in C and D. WT controls: 21.09 ± 1.045 (n = 4 animals, 15 retinal regions); Fat3∆TM/∆TM: 21.59 ± 0.969 (n = 4 animals, 15 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (G) Examples of retinal sections showing cells expressing GFP under the control of the Grik1 enhancer. The GFP reporter was introduced through in vivo injection of AAV8-Grik1-GFP. WT retinas are shown in G. (H)Fat3∆TM/∆TM retinas injected with AAV8-Grik1-GFP. The OPL is indicated with a yellow arrowhead and the OMPL, labeled with VGAT staining, is marked with a yellow arrow. Scale bar: 20 µm. (I) Quantification of Grik1+ BC dendrites in the OPL (WT Controls: 100%; Fat3∆TM/∆TM: 100%), axons in the IPL only (WT Controls: 100%; Fat3∆TM/∆TM: 44 ± 18.6%), and axons in the OMPL or in the OMPL and IPL (WT Controls: 0%; Fat3∆TM/∆TM: 56 ± 18.6%). Controls: n = 5 animals, 12 cells, Fat3∆TM/∆TM: n = 7 animals, 22 cells. Mann–Whitney test. Error bars: SEM.
Figure S4.

BC and cones numbers, visualization of Grik1+ BCs morphology via injection of an AVV-Grik1-GFP virus in Fat3 mutants (Related to Figs. 3 and 4). (A) ARR3 immunohistochemistry of WT retinas. (A′) shows ARR3 staining together with DAPI staining. (B) ARR3 immunohistochemistry of Fat3∆TM/∆TM retinas. (B′) shows ARR3 staining together with DAPI staining. (C) VSX2 immunostaining of WT retinas. (D) VSX2 immunostaining of Fat3∆TM/∆TM retinas. (E) Quantification of number of cones marked by ARR3, as shown in A and B. WT controls: 27.77 ± 0.907 (n = 4 animals, 13 retinal regions); Fat3∆TM/∆TM: 29.00 ± 0.593 (n = 4 animals, 14 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (F) Quantification of thickness of area occupied by VSX2 staining, as shown in C and D. WT controls: 21.09 ± 1.045 (n = 4 animals, 15 retinal regions); Fat3∆TM/∆TM: 21.59 ± 0.969 (n = 4 animals, 15 retinal regions). Each data point corresponds to a retinal region, color-coded by animal, analyzed using a nested two-tailed test. (G) Examples of retinal sections showing cells expressing GFP under the control of the Grik1 enhancer. The GFP reporter was introduced through in vivo injection of AAV8-Grik1-GFP. WT retinas are shown in G. (H)Fat3∆TM/∆TM retinas injected with AAV8-Grik1-GFP. The OPL is indicated with a yellow arrowhead and the OMPL, labeled with VGAT staining, is marked with a yellow arrow. Scale bar: 20 µm. (I) Quantification of Grik1+ BC dendrites in the OPL (WT Controls: 100%; Fat3∆TM/∆TM: 100%), axons in the IPL only (WT Controls: 100%; Fat3∆TM/∆TM: 44 ± 18.6%), and axons in the OMPL or in the OMPL and IPL (WT Controls: 0%; Fat3∆TM/∆TM: 56 ± 18.6%). Controls: n = 5 animals, 12 cells, Fat3∆TM/∆TM: n = 7 animals, 22 cells. Mann–Whitney test. Error bars: SEM.

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