Scotopic and photopic ERG of FAT3-deficient mice (Related toFig. 1). (A) Representative OCT images of Fat3∆TM/+ control and Fat3∆TM/∆TM eyes. (B) Representative scotopic ERG raw traces of Fat3∆TM/+ and Fat3∆TM/∆TM eyes elicited by 0.1 cd s/m2 flashes. (C) Quantification of scotopic ERG parameters (amplitude and implicit time of a-wave and b-wave) of Fat3∆TM/+ (n = 8) and Fat3∆TM/∆TM (n = 10) eyes. (D) Representative photopic ERG raw traces of Fat3ΔTM/+ and Fat3ΔTM/ΔTM eyes elicited by 1, 10, 100, and 1,000 cd s/m2 flashes at 30 cd/m2 background light to saturate the response from rod-pathway. (E) Ensemble-averaged photopic ERG b-wave amplitude from Fat3∆TM/+ (n = 8) and Fat3∆TM/∆TM (n = 10) eyes. (F) Schematics of fear conditioning and optomotor behavioral experiment. On Day 1, a mouse is brought to the electric-shock cage with a floor of metal bars for habituation of the environment. On Day 2, the mouse is conditioned by electrical shock paired with 33 Hz flashing light. On Day 3, the mouse is first subjected to a contextual check, in which the “Context” measures the freezing time of the mouse after it is brought back to the electric shock cage, which presents a fear-associated context environment, without the shock. “Static” measures the freezing time of the mouse with a static light, after the covering the metal bars and an odor change. Following this measurement, a 33 Hz flickering light is turned on, and the freezing time of the mouse is measured, as the “flicker” time. (G) Fear conditioning responses as freezing time (seconds) from Fat3∆TM/+ (n = 8) and Fat3∆TM/∆TM (n = 9) mice. One-way ANOVA (matched data) with Dunnett multiple comparison test. (H) The visual threshold of spatial frequency of WT (n = 8) and Fat3∆TM/∆TM (n = 10) mice measured with the optomotor behavioral assay shown in the bottom cartoon in panel D. Unpaired two-tailed Student’s t test. Abbreviations: RPE, retinal pigmented epithelium; IS/OS, inner-outer segments junction; OLM, outer limiting membrane; OPL, outer plexiform layer; IPL, inner plexiform layer; IMPL: inner misplaced plexiform layer. NS: non-significant. Error bars: SEM.
Figure S1.

Scotopic and photopic ERG of FAT3-deficient mice (Related to Fig. 1 ). (A) Representative OCT images of Fat3∆TM/+ control and Fat3∆TM/∆TM eyes. (B) Representative scotopic ERG raw traces of Fat3∆TM/+ and Fat3∆TM/∆TM eyes elicited by 0.1 cd s/m2 flashes. (C) Quantification of scotopic ERG parameters (amplitude and implicit time of a-wave and b-wave) of Fat3∆TM/+ (n = 8) and Fat3∆TM/∆TM (n = 10) eyes. (D) Representative photopic ERG raw traces of Fat3ΔTM/+ and Fat3ΔTM/ΔTM eyes elicited by 1, 10, 100, and 1,000 cd s/m2 flashes at 30 cd/m2 background light to saturate the response from rod-pathway. (E) Ensemble-averaged photopic ERG b-wave amplitude from Fat3∆TM/+ (n = 8) and Fat3∆TM/∆TM (n = 10) eyes. (F) Schematics of fear conditioning and optomotor behavioral experiment. On Day 1, a mouse is brought to the electric-shock cage with a floor of metal bars for habituation of the environment. On Day 2, the mouse is conditioned by electrical shock paired with 33 Hz flashing light. On Day 3, the mouse is first subjected to a contextual check, in which the “Context” measures the freezing time of the mouse after it is brought back to the electric shock cage, which presents a fear-associated context environment, without the shock. “Static” measures the freezing time of the mouse with a static light, after the covering the metal bars and an odor change. Following this measurement, a 33 Hz flickering light is turned on, and the freezing time of the mouse is measured, as the “flicker” time. (G) Fear conditioning responses as freezing time (seconds) from Fat3∆TM/+ (n = 8) and Fat3∆TM/∆TM (n = 9) mice. One-way ANOVA (matched data) with Dunnett multiple comparison test. (H) The visual threshold of spatial frequency of WT (n = 8) and Fat3∆TM/∆TM (n = 10) mice measured with the optomotor behavioral assay shown in the bottom cartoon in panel D. Unpaired two-tailed Student’s t test. Abbreviations: RPE, retinal pigmented epithelium; IS/OS, inner-outer segments junction; OLM, outer limiting membrane; OPL, outer plexiform layer; IPL, inner plexiform layer; IMPL: inner misplaced plexiform layer. NS: non-significant. Error bars: SEM.

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