Flicker ERG and vision at high frequency and step ERG of Fat3-deficient mice. (A) Schematic representation of retinal neurons and their layers. (B) Averaged oscillatory potentials of Fat3^∆TM/+ (n = 8) and Fat3^∆TM/∆TM (n = 10) eyes at scotopic condition are elicited by 0.1 cd s/m² flashes (same as Fig. S1 B). Line: Mean values. Error bar: SEM. (C) Peak amplitude (N3-P3) of oscillatory potentials (same as Fig. 1 B, 0.1 cd s/m² scotopic condition). Fat3^∆TM/+: 146.7 ± 16.8 µV, n = 8 eyes; Fat3^∆TM/∆TM: 179.9 ± 26.5 µV, n = 10 eyes. Unpaired two-tailed Student’s t test. Error bar: SEM. The numerical values above the bar graph for this and the rest of figures: P values. (D) Peak implicit time (P3) of oscillatory potentials. Fat3^∆TM/+: 34.38 ± 0.18 ms, n = 8 eyes; Fat3^∆TM/∆TM: 33.70 ± 0.37 µV, n = 10 eyes. Unpaired two-tailed Student’s t test. Error bar: SEM. (E) Representative flicker ERG raw traces of WT control and Fat3^∆TM/∆TM eyes elicited by 3.162 cd s/m² flashes at 20, 30, 40, and 50 Hz frequencies. (F) Flicker ERG amplitude at 0.5, 10, 20, 30, 40, and 50 Hz for WT control (n = 10) and Fat3^∆TM/∆TM (n = 10) eyes. Unpaired two-tailed Student’s t test. Error bar: SEM. (G) Flicker ERG implicit time (first peak) at 20 Hz for WT control (36.5 ± 0.5 ms, n = 10) and Fat3^∆TM/∆TM (46.2 ± 1.0 ms, n = 10) eyes. Unpaired two-tailed Student’s t test. Error bar: SEM. (H) Representative step ERG raw traces of WT (n = 10) control and Fat3^∆TM/∆TM (n = 10) eyes elicited by a 3-s step light at 1,000 cd/m² intensity. (I) Quantification of step ERG d-wave amplitudes of WT (38.9 ± 2.2 µV, n = 10) control and Fat3^∆TM/∆TM (30.2 ± 3.0 µV, n = 10) eyes elicited by a 3-s step of light at 1,000 cd/m² intensity. Unpaired two-tailed Student’s t test. Error bar: SEM.