Quantitative real-time PCR analysis of purified Tomato ⁺ cells. (A–D) Real-time PCR analysis of mRNA levels of Reln (A), Myh11 (B), Cd34 (C), and Col1a1 (D) in purified Tomato⁺CD45⁻ liver cells from mice of the indicated genotype. The statistical significance of differences was assessed by two-tailed Student’s t tests. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 5 mice per genotype from three independent experiments). (E) H&E staining of liver sections from Cd34^creER;R26^DTA and R26^DTA mice at 2 wk after tamoxifen treatment. Scale bar = 100 µm.