scRNA-seq analysis of non-parenchymal liver cells. (A) Visualization of unsupervised clustering in a t-SNE plot of liver cells from Dobie et al. (2019) and Wang et al. (2021). (B) Heatmap showing the top 100 differentially expressed genes in each cell cluster. (C and D) Violin plots showing the expression levels of Myh11 (C) and Reln (D) in different cell clusters.