Rab11 interacts with MICAL1 to promote F-actin depolymerization. (A) Immunofluorescence images for RPE1 cells transfected with control or TBC1D20 siRNAs, cultured in serum medium, and stained with the antibodies against CEP164 and Rab35. (B) Immunofluorescence images for RPE1 GFP-Rab35 cells transfected with control or TBC1D20 siRNAs, cultured in serum medium, and stained with the antibody against CEP164. (C) Immunofluorescence images for RPE1 tRFP-Rab8a cells transfected with control or TBC1D20 siRNAs, cultured in serum medium, and stained with the antibody against CEP164. (D and E) Immunofluorescence images (D) and quantification of the fluorescence intensity of transferrin (E) for RPE1 GFP-Rab11B cells transfected with control or TBC1D20 siRNA, cultured in serum-free medium, and stained with the antibody against EEA1. n > 30 cells. (F and G) Immunofluorescence images (F) and quantification of the fluorescence intensity of transferrin (G) for RPE1 cells transfected with control or TBC1D20 siRNA, cultured in serum-free medium, and stained with the antibodies against CEP164 and EEA1. n > 30 cells. All experiments were repeated at least three times. Scale bar, 5 µm.