Temporal evolution of response to TNFα. Mice were injected i.v. with TNFα, and splenic monocytes (Mo) and peritoneal macrophages (MF.PC) were profiled by population RNAseq at different times. Two mice were pooled for each biological replicate, for two biological replicates per condition. (A) Overview count of upregulated and downregulated genes (FoldChange >2 [top] and <0.5 [bottom], with nominal P value <0.01). (B) Heatmap of the response to TNFα, with genes arranged in the same order as Fig. 1 B. (C) Quantification of normalized gene counts of inflammatory cytokine transcripts (Il1a, IL1β, Il6, and TNFα) in Mo and MF.PC during the course of response to TNFα (PBS-injected controls sampled at 24 h). (D) Clustered heatmap of 944 genes for Mo (left) and 1,079 genes for MF.PC (right) (FoldChange >2 [up] or <0.5 [down], and P value <0.01 in at least one time point for each cell type). (E) FoldChange/FoldChange plot comparing the effects of TNFα in Mo (x-axis) and MF.PC (y-axis). G2/M signature genes are highlighted in purple.