Early chromatin changes around genes responsive to IL1β in neutrophils. Chromatin analysis was performed by CUT&RUN in neutrophils (GNs) purified from mice at baseline or 3 h after IL1β treatment in vivo. (A) Scatterplots comparing normalized reads in peak signals between untreated GNs (summed signals of GNs from bone marrow, spleen, blood), and IL1β-treated GNs (summed from GNs in lung and peritoneal cavity) samples, averaged between female and male replicates. Peaks associated with the top 50 upregulated (red) and downregulated (blue) genes in GNs (from Fig. 2) are highlighted. (B) Representative CUT&RUN chromatin tracks around IL1β-induced genes, Cd14 and Socs3, and IL1β-repressed genes, Atf3 and Trim65, for the major histone marks or from non-specific rabbit IgG controls. FC, FoldChange.