Overview of total early transcriptional profiles of immune cells in response to IL1β, IL6, and TNFα. Mice were injected systemically with inflammatory cytokines, and the main immunocyte populations were sorted 2 h later and profiled by ultra-low input population RNAseq. See Materials and methods for information on replicates. (A) A clustered heatmap overview of 2,331 genes that respond in one or more cell types across IL1β, IL6, and TNFα. (B) Gene ontology (GO) analysis (String) incorporating all up- or downregulated genes from A. Only a representative subset of the 76 ontologies passing P_adj <0.05 are shown for upregulated transcripts, but all are shown for downregulated genes. (C) Genes belonging to the main categories of enriched Gene Ontologies within the upregulated transcripts of A. (D) Enrichment of TFs controlling responsive clusters was analyzed for each cluster from A using the “ENCODE and ChEA Consensus TFs from ChIP-X” library from EnrichR. TF motifs yielding the 10 best “Combined Score” were included. Individual scores are shown (full listing and significance metrics in Table S2). In some instances (Runx, IRF, and NF-κB), motifs were edited to the TF family name rather than the individual member predicted by EnrichR.