Overview of early responses to IL1β, IL6, and TNFα. (A) Schematic of IL1β, IL6, and TNFα signaling pathways. Solid lines represent pathways canonically considered to dominate the responses, dotted lines represent pathways considered more peripheral. Created with https://BioRender.com. (B) Mice were injected systemically with inflammatory cytokines, the main immunocyte populations were sorted 2 h later and profiled by population RNAseq. Numbers tally the upregulated and downregulated genes (FoldChange >2 [top] or <0.5 [bottom], P value <0.001). See Materials and methods for information on replicates.