Murine CD8 ⁺ TIL phenotyping and human XCL1/XCL2 expression profile. (A) Experiment setup in which preactivated P14 T cells were transferred into tumor-bearing mice, followed by tissue analysis D7 after transfer. (B) Representative plots of P14 in indicated tissue, subset label shown, n = 5. (C) Representative histograms of marker expression within the indicated subset defined by TCF1 and TIM3. (D) qPCR validation of Xcl1 overexpression in P14 T cells. (E) Tumor growth curves of mice receiving no T cells, CD8^EV, and CD8^Xcl1. Each line represents an individual mouse. (F) Mean expression of XCL1 and XCL2 across annotated clusters within five human tumor types. Clusters with high expression (Fig. 4 F) are highlighted in blue. (G) Top 20 genes with similar expression profile to XCL1 in SKCM. Genes of interest are highlighted in blue (GEPIA 2.0). Data representative of at least two independent experiments (B–E). P value shown, unpaired t test (D).