Confirmation of XCR1 ⁺ depletion and whole tissue microscopy. (A) CRISPR targeting efficiency (TIDE) and Xcl1 qPCR data. (B) Quantification of CD69⁻ CD103⁻ population in IEL and LP D25 following LCMV-Arm. (C) Experiment setup in which naïve P14 T cells transferred into WT or XCR1^Ven/Ven (left). Subset frequencies of IEL and LP P14 T cells and cell numbers. (D) Confocal imaging validation of XCR1⁺ cell depletion in SI following DT treatment. Marker colors indicated in panel. (E) Quantification of IEL CD103⁺ P14 T cell frequency at D8, each dot represents one mouse. (F)Xcl1 gene expression in SI P14 T cells during LCMV-Arm timecourse. (G) Spatial transcriptomic mean RNA expression of Cxcr3 and Ccr9 P14 T cells. P value shown, unpaired t test (A–C and E) and two-way ANOVA (C and G).