Off-gating current kinetics for WT, IQM, and CW with different depolarization duration. (A–C) Off-gating current from WT, IQM, and CW with 2 ms (red), 10 ms (blue), and 40 ms (black) depolarization. Note, only in CW, the fast component of off-gating current is getting slower. Inset shows the voltage protocol. Current is normalized to the peak. (D–F) fast time constant of WT, IQM, and CW characterized with different depolarization durations. In CW, the kinetics become significantly slower with longer depolarization. This is not seen in either WT or IQM. Data plotted are Mean ± SEM. N = 6 for WT, N = 7 for IQM, and N = 6 for CW.