Gating current for WT, IQM, and CW. (A) Example on-gating current traces for WT, IQM, and CW. Inset show the voltage protocol. (B) QV curves for WT, IQM, and CW were obtained by integrating the off-gating current after 30 ms. Compared with WT, IQM, and CW both have a right-shifted QV curve. N = 6 for WT and CW, N = 5 for IQM. Data plotted as MEAN ± SEM. (C) On-gating current kinetics for WT, IQM, and CW. On-gating current was fitted with a two-component exponential function. (D) Example of off-gating current for WT, IQM, and CW. Inset shows the voltage protocol. (E) Off-gating kinetics fitted with two exponential decay components. Note that in CW, the fast component is significantly slower when compared with WT. N = 7 for WT, N = 6 for CW and N = 8 for IQM.