Binding of the IFM motif drives CW into an alternative open state. (A) ATX-II has minimal effect on CW_IQM unlike CW. Inset shows the voltage protocol. (B and C) GV curves for CW_IQM normalized to the maximum conductance after ATX-II treatment (B) or to the maximum conductance in each condition (C). N = 4. (D and E) Tail current with different depolarization times. Tail current was measured with difference depolarization time in (D) CW and (E) IQM. The inset shows the voltage protocol. (F) Tail current kinetics characterized as a function of depolarizing time. Note in CW, the tail current became slower and slower as depolarization time increases. Data plotted as Mean ± SEM. N = 5 for both cases.