Phenotypes of CW_IQM. (A) Example ionic current traces for CW_IQM. Fast inactivation is completely removed. The inset shows voltage protocol. (B) GV curve for CW_IQM. Surprisingly, the voltage dependence of CW_IQM activation is significantly shifted to the left for around 15 mV compared with the WT, similar to the ATX-II treated CW. This shift in the GV curve is not observed in either IQM or CW, suggesting two mutations interact. N = 4. Data plotted as mean ± SEM. (C) Tail-current kinetics of CW_IQM compared with WT, IQM, and CW. N = 5.