Functional measurements for mutations in VDS _I and VSD _II R2 to glutamine. (A) Representative currents for each channel in response to a series of 100-ms voltage pulses from −90 to 70 mV in 10 mV increments. (B) Conductance versus voltage plots comparing WT (n = 9) to R222Q (n = 5) and R811Q (n = 6). (C) Steady-state channel availability curves comparing WT (n = 8) to R222Q (n = 7) and R811Q (n = 7). (D) Plots showing the recovery time from inactivation again comparing WT (n = 7) to R222Q (n = 7) and R811Q (n = 8). Fits were performed as described in the Materials and methods and the Results are shown in Table S2 along with the determination of significance using a one-way ANOVA with a post hoc Dunnett’s test.