Bulk RNA sequencing of SV40-fibroblasts. (A) Heatmap showing the normalized and scaled expression (vst) of genes significantly upregulated in control SV40-fibroblasts after stimulation with TNFα 10 ng/ml for 6 h and significantly downregulated in SV40-fibroblasts from the patient compared with the controls after TNFα stimulation (from the TNFα via NF-κB signaling list of genes, MSIgDB). Each column represents a patient or a control. RNA sequencing has been performed in biological duplicates for each control or patient (as indicated by _1 or _2 at the end of each column name). (B) Heatmap showing the normalized and scaled expression (vst) of genes significantly upregulated in control SV40-fibroblasts after stimulation with Poly I:C 1 µg/ml for 6 h from the TNFα via NF-κB signaling list of genes, MSIgDB. Each column represents a patient or a control. RNA sequencing has been performed in biological duplicates for each control or patient (as indicated by _1 or _2 at the end of each column name). (C) Heatmap showing the list of genes significantly downregulated in patient’s cells compared to control cells after poly I:C 1 µg/ml 6 h stimulation, from the list of genes TNFα via NF-κB signaling list of genes, MSIgDB. (D) Z-score of the different “molecules” predicted to be inhibited (top 10 based on the z-score) based on IPA (Qiagen Inc.) after poly I:C stimulation. A negative score indicate that the molecule is predicted to be inhibited based on the DEGs that have been processed.