Exploration of the canonical NF-κB signaling in patient cells. (A) Heatmap showing the normalized and scaled expression (vst) of genes significantly upregulated in control SV40-fibroblasts after stimulation with TNFα 10 ng/ml for 6 h. Each column represents a patient or a control. RNA sequencing has been performed in biological duplicates for each control or patient (as indicated by _1 or _2 at the end of each column name). (B) Pathway enrichment score of DEGs between the patient and controls SV40-fibroblasts stimulated with TNFα. A negative blue bar indicates a downregulation of the indicated pathway based on the PROGENy database while a red bar indicates an upregulated pathway. (C) Z-score of the different “molecules” predicted to be inhibited (top 10 based on the z-score) based on IPA (Qiagen Inc.) for the mentioned conditions. A negative score indicate that the molecule is predicted to be inhibited based on the DEGs that have been processed. The RNA sequencing experiment has been performed once in duplicate.