Figure 5.
Non-canonical NF-κB signaling in patient cells. (A) Western blot of NFκB2 p100/p52 on cell lysates from SV40-fibroblasts after activation of the non-canonical pathway with LTαβ for 8, 24, or 48 h, as indicated. β-Actin was used as a loading control. Representative of at least three experiments. *: P-value <0.05 after Mann-Whitney Wilcoxon test. (B) Western blot of NFκB2 p100/p52 on cell lysates from activated T cells starved 24 h before stimulation with OKT3 for 48 h. β-Actin was used as a loading control. Representative of two experiments. (C) Western blot of cytoplasmic and nuclear protein extracts from SV40-fibroblasts activated with LTαβ for 24 h assessing the translocation of RELB in the nucleus. α-Tubulin was used the loading control of cytoplasmic extracts while Lamin A/C was used for nuclear extracts. Representative of three experiments. Source data are available for this figure: SourceData F5.

Non-canonical NF-κB signaling in patient cells. (A) Western blot of NFκB2 p100/p52 on cell lysates from SV40-fibroblasts after activation of the non-canonical pathway with LTαβ for 8, 24, or 48 h, as indicated. β-Actin was used as a loading control. Representative of at least three experiments. *: P-value <0.05 after Mann-Whitney Wilcoxon test. (B) Western blot of NFκB2 p100/p52 on cell lysates from activated T cells starved 24 h before stimulation with OKT3 for 48 h. β-Actin was used as a loading control. Representative of two experiments. (C) Western blot of cytoplasmic and nuclear protein extracts from SV40-fibroblasts activated with LTαβ for 24 h assessing the translocation of RELB in the nucleus. α-Tubulin was used the loading control of cytoplasmic extracts while Lamin A/C was used for nuclear extracts. Representative of three experiments. Source data are available for this figure: SourceData F5.

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