Co-immunoprecipitation between IKKα and NIK. (A) Whole cell lysate of HEK293T cells co-transfected with Flag-NIK and EV or HA-IKKa wt or mutants (as indicated) were subjected to western blot. Immunoblot of Flag-NIK, HA-IKKα, and vinculin as a housekeeping gene. (B) Immunoprecipitation of Flag-NIK after transfection of HEK293T cells with Flag-NIK and HA-IKKα wt or mutants (as indicated). Immunoblot of Flag-NIK and HA-IKKα. All figures are representative of at least two independent experiments. Both panels are representative of two independent experiments. Source data are available for this figure: SourceData F4.