Schematics of the domain structure of BAG6 and its derivative architecture designed in this study. (A) Schematic of the human FL form of BAG6 protein (NP_001092004.1). The positions of Domains I (N200) and other domains are indicated. Numbers denote the corresponding amino acid positions. (B) Schematic of the domain structure of the TanGIBLE developed in this study. Domain I is composed of two highly conserved regions, UBL (indicated by violet squares) and BUILD (indicated by red squares). Note that TanGIBLE consists of two Domain I units tandemly connected with a glycine (Gly) linker. N-terminal hydrophilic tags are shown in green. (C) TanGIBLE is composed of four tandemly aligned ordered domains. The amino acid sequence of TanGIBLE was analyzed using the disorder/order structure prediction programs PONDR. Stretches corresponding to the UBL sequence are indicated with a blue box and those corresponding to the BUILD sequence are indicated with a red box. The results from two different predictor packages, VSL2 (violet line) and VL3 (blue line), are shown.