Immunofluorescent imaging and LCMS of stable Tg:hsp70:mybpc2b-FLAG transgenic zebrafish larvae (F3 generation) and sibling wildtype controls after heat shock treatment (see Materials and methods). (A, inset) Confocal image of heat-shocked and anti-FLAG immunostained Tg:hsp70:mybpc2b-FLAG 5 dpf larval tail shows widespread “doublet” staining pattern. (B) LCMS of 5 dpf heat shocked Tg(hsp70:mybpc2b-FLAG) larval tails shows no significant increase in accumulation of Mypbc2b peptides versus wildtype.