Impairment of dcLN CSF outflow recruits the nasal-to-scLN pathway. (A) Schematic of experimental conditions. (B) Representative images of dcLN and scLN sections showing tracer drainage across ligation conditions (scale = 200 µm, n = 6, 5, 6, 6; scale = 100 µm). (C) Quantification of dcLN tracer drainage (ANOVA: P = 0.00073, Tukey HSD: *P < 0.05, **P < 0.01). (D) Quantification of scLN tracer drainage (ANOVA: P = 0.000083, Tukey HSD: ***P < 0.001). (E) Representative sagittal projections of light-sheet imaging of tracer distribution from cleared skulls (n = 6, 5, 6, 6; scale = 1 mm). (F) Quantification of E (ANOVA: P = 0.0034, Tukey HSD: *P < 0.05, **P < 0.01). (G) Representative horizontal view of tracer distribution in skulls after dissection (n = 6, 5, 6, 6; scale = 500 µm). (H) Quantification of G (ANOVA: P = 0.0000073, Tukey HSD: *P < 0.05, **P < 0.01, ****P < 0.0001).
Figure 3.

Impairment of dcLN CSF outflow recruits the nasal-to-scLN pathway. (A) Schematic of experimental conditions. (B) Representative images of dcLN and scLN sections showing tracer drainage across ligation conditions (scale = 200 µm, n = 6, 5, 6, 6; scale = 100 µm). (C) Quantification of dcLN tracer drainage (ANOVA: P = 0.00073, Tukey HSD: *P < 0.05, **P < 0.01). (D) Quantification of scLN tracer drainage (ANOVA: P = 0.000083, Tukey HSD: ***P < 0.001). (E) Representative sagittal projections of light-sheet imaging of tracer distribution from cleared skulls (n = 6, 5, 6, 6; scale = 1 mm). (F) Quantification of E (ANOVA: P = 0.0034, Tukey HSD: *P < 0.05, **P < 0.01). (G) Representative horizontal view of tracer distribution in skulls after dissection (n = 6, 5, 6, 6; scale = 500 µm). (H) Quantification of G (ANOVA: P = 0.0000073, Tukey HSD: *P < 0.05, **P < 0.01, ****P < 0.0001).

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