Postural differences control nasal-to-scLN CSF outflow through ICP changes. (A) Schematic of experimental conditions. (B) Mean ICP in head-up, level, and down positions (n = 3, one-way repeated-measures ANOVA: P = 0.0004, pairwise, paired t tests (Holm’s method): *P < 0.05, **P < 0.01). (C) Representative images of dcLN and scLN sections showing tracer drainage across posture conditions (n = 6, 6, 12, 11; scale = 200 µm). (D) Quantification of dcLN tracer drainage (ANOVA: P = 0.057). (E) Quantification of scLN tracer drainage (ANOVA: P = 0.00007, Tukey HSD: **P < 0.01, ***P < 0.005). (F) Representative horizontal and sagittal projections of light-sheet imaging of tracer distribution from cleared skulls (n = 5, 5; scale = 1 mm). (G) Quantification of F (Student’s t test, *, P = 0.046). (H) Representative horizontal view of tracer distribution in skulls after dissection (n = 6, 5, scale = 500 µm). (I) Quantification of H (Student’s t test, *, P = 0.037).
Figure 2.

Postural differences control nasal-to-scLN CSF outflow through ICP changes. (A) Schematic of experimental conditions. (B) Mean ICP in head-up, level, and down positions (n = 3, one-way repeated-measures ANOVA: P = 0.0004, pairwise, paired t tests (Holm’s method): *P < 0.05, **P < 0.01). (C) Representative images of dcLN and scLN sections showing tracer drainage across posture conditions (n = 6, 6, 12, 11; scale = 200 µm). (D) Quantification of dcLN tracer drainage (ANOVA: P = 0.057). (E) Quantification of scLN tracer drainage (ANOVA: P = 0.00007, Tukey HSD: **P < 0.01, ***P < 0.005). (F) Representative horizontal and sagittal projections of light-sheet imaging of tracer distribution from cleared skulls (n = 5, 5; scale = 1 mm). (G) Quantification of F (Student’s t test, *, P = 0.046). (H) Representative horizontal view of tracer distribution in skulls after dissection (n = 6, 5, scale = 500 µm). (I) Quantification of H (Student’s t test, *, P = 0.037).

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