Supplemental measurements of contractile pumping and extrinsic forces acting on lymphatic outflow to the scLN and dcLN . (A) Peak amplitude in lymphatic contractility in the typical range of 2–20 min⁻¹ (µm deviation from mean diameter, Lomb-Scargle periodogram, n = 10, 8, Student’s t test, ****, P = 0.0001). (B) Amplitude of mean max-min vessel diameter in lymphatic contractility of scLN and dcLN afferents (µm deviation from mean diameter, n = 10, 8, Student’s t test, ***, P = 0.0001). (C) Mean diameter of measured lymphatic vessels (n = 10, 8, Student’s t test, P = 0.79). (D) Representative Lomb-Scargle periodograms of dcLN-afferent cLV particle flow oscillation showing a peak in respiratory frequency range (mean 137.6 ± 3.3 min⁻¹). (E) Typical mouse respiratory rates (RR, 172 ± 26 min⁻¹) and heart rates (HR, 341 ± 31 min⁻¹) measured under ketamine/xylazine anesthesia (n = 5). (F) Representative ICP trace showing cardiorespiratory oscillation in the waveform (n = 3). (G) Lomb-Scargle periodogram of F showing respiratory (RP, 174 ± 4.6) and cardiac (CP, 348 ± 9.2) frequency peaks. (H) Lomb-Scargle periodograms showing valve-indicated flow oscillation in the frequency range of lymphatic pumping for scLN afferents and dcLN afferents with false alarm probability <0.05 highlighted by −log(p) (n = 4, 3). (I) Lomb-Scargle periodograms showing valve-indicated flow oscillation in the frequency range of the respiratory rate for scLN afferents and dcLN afferents with false alarm probability <0.05 highlighted by −log(p) (n = 4, 3). (J) Frequency of spectral amplitude peaks in LMC GCaMP6f fluorescent signal in the range of 0-20 min⁻¹ (Lomb-Scargle periodogram, n = 3, 3, Student’s t test, *, P = 0.024). (K) Peak amplitude of LMC GCaMP6f fluorescent signal of scLN and dcLN afferents (percent mean diameter, n = 3, 3, Student’s t test, *, P = 0.048). (L) Peak amplitude of LMC GCaMP6f vessel diameter in scLN and dcLN afferents (percent mean diameter, n = 3, 3, Student’s t test, *, P = 0.017).