The mechanism of MYO18B promoting lysosomal exocytosis. MYO18B, as an actin crosslinker, promotes the assembly of stress fibers, which in turn facilitates FA maturation. Lysosomes moving along microtubules are transported to FAs, as a result of the intimate interaction between microtubule and FA mediated by CLASPs and other adaptor proteins. The contractile force generated by stress fiber is conveyed to the plasma membrane at around FAs which causes an increased membrane tension and activates mechanosensitive cation channel PIEZO1. The Ca²⁺ imported into the cell by PIEZO1 triggers lysosome-plasma membrane fusion. Paucimannose, a series of truncated N-glycan mainly generated in lysosomes, is secreted through this pathway.