The N-terminal extension of MYO18B is critical for its role in lysosomal exocytosis. (A) Schematic representation of different MYO18B truncated constructs. (B) Western blot of all MYO18B truncates. (C) Representative plasma membrane repairing assay results of WT, MYO18B-KO, and MYO18B-KO cells stably transfected with different MYO18B truncates. (D) HEX secretion of WT, MYO18B-KO, and MYO18B-KO cells stably transfected with different MYO18B truncates. Data are the mean ± SD from three independent experiments (One-way ANOVA with Dunnett correction; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, P ≥ 0.05). See also Fig. S2 D. (E) Representative CLSM images of WT, MYO18B-KO, and MYO18B-KO cells stably transfected with different MYO18B truncates stained with vinculin. Bar, 10 µm. See also Fig. S2 E. (F) Quantitative results of number of FAs > 0.5 µm² (mean ± SD; n = 10 images; N = 2 biological replicates; One-way ANOVA with Dunnett correction; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, P ≥ 0.05). Source data are available for this figure: SourceData F5.