Figure S3.
Transmission electron microscopy analysis of HeLa cell knocked out for ATG9A or knocked down for CHMP2A and IQGAP1. (A) Quantification of the average number of autophagosomes (black bars) and phagophores (gray bars) per cell section in HeLaWT or HeLaATG9AKO, HeLa siRNA control (siScr), or HeLa knockdown for CHMP2A and IQGAP1 (non-normalized data corresponding to the graph in Fig. 3 D). (B and C) Electron micrographs as in Fig. 3 with highlighted (yellow) open regions. (C and D) Additional examples (micrographs) representing phagophores containing mitochondria or not in CHMP2A and IQGAP1 knockdown cells quantified in Fig. 3 D. T test. Data means ± SE. Scale Bar 500 nm. A: Autophagosome; ER, Endoplasmic Reticulum; M, Mitochondria; M*, Mitochondria inside a phagophore; N, Nucleus; P, Phagophore. Source data are available for this figure: SourceData FS3.

Transmission electron microscopy analysis of HeLa cell knocked out for ATG9A or knocked down for CHMP2A and IQGAP1. (A) Quantification of the average number of autophagosomes (black bars) and phagophores (gray bars) per cell section in HeLaWT or HeLaATG9AKO, HeLa siRNA control (siScr), or HeLa knockdown for CHMP2A and IQGAP1 (non-normalized data corresponding to the graph in Fig. 3 D). (B and C) Electron micrographs as in Fig. 3 with highlighted (yellow) open regions. (C and D) Additional examples (micrographs) representing phagophores containing mitochondria or not in CHMP2A and IQGAP1 knockdown cells quantified in Fig. 3 D. T test. Data means ± SE. Scale Bar 500 nm. A: Autophagosome; ER, Endoplasmic Reticulum; M, Mitochondria; M*, Mitochondria inside a phagophore; N, Nucleus; P, Phagophore. Source data are available for this figure: SourceData FS3.

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