PtdIns(3,5)P2 binding proteins in T cell lysates and kinase motif analysis of phosphosites impacted by apilimod treatment. (A) Examples of proteins in the PtdIns(3,5)P2 bead binding assay are highlighted for mTOR signaling, TCR signaling, PKA signaling, glycolysis, and kinases. (B) Kinase motifs that were impacted by apilimod in the phosphoproteomic analysis were identified using the NetworKin software package.