Figure S2.

GJA1-20k without RPEL motif results in less actin puncta formation compared with GJA1-20k. (A) Representative images of actin puncta formation in HeLa cells transfected with either GJA1-20k or GJA1-20kΔαCT11. Scale bars = 20 and 5 µm (inset). (B) Quantification of actin puncta density in A (n = 113, 127 cells, GJA1-20k, and GJA1-20kΔαCT11, respectively, from three independent experimental repeats). Data are presented as mean ± SD. ****P < 0.0001 with two sample Mann–Whitney U test.

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