Hierarchical relationships between MEL-28 ^ELYS and nucleoporins bearing a membrane-binding domain during interkinetic envelope assembly. Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and either GFP-tagged NPP-6^NUP160, NPP-15^NUP133, NPP-25^TMEM33, NPP-12^NUP210, NPP-19^NUP35, or NPP-7^NUP153 (green) during interkinesis and interphase in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the normalized GFP-tagged NPP-6^NUP160, NPP-15^NUP133, NPP-25^TMEM33, NPP-12^NUP210, NPP-19^NUP35, or NPP-7^NUP153 integrated intensity over time from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue and mel-28(RNAi) in purple. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann–Whitney test on the mean value of GFP::LEM-2^LEMD2/3 intensity in interkinesis (**P < 0.01, ****P < 0.0001).