Nucleoporins with potential membrane-binding domains could contribute to interkinetic envelope integrity but not to chromosome segregation. (A) Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::LEM-2^LEMD2/3 (green) during meiosis I and II in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the GFP::LEM-2^LEMD2/3 integrated intensity normalized over a background from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue, npp-8(RNAi) in yellow. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann–Whitney test on the mean value of GFP::LEM-2^LEMD2/3 intensity in interkinesis. (B) Quantification of the distance between the two sets of segregating chromosomes over time from anaphase I onset. Control in dark blue, npp-6^NUP160(RNAi) in turquoise, npp-25^TMEM33(RNAi) in dark orange, npp-15^NUP133(RNAi) in light orange, npp-12^NUP210(RNAi) in gray, npp-19^NUP53(RNAi) in pink and npp-7^NUP153(RNAi) in light brown. Mann–Whitney test on the mean distance between the segregating chromosomal sets during interkinesis in both conditions. (C) Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::LEM-2^LEMD2/3 (green) during interkinesis and interphase in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm.