Depletion efficiency of various nucleoporins by RNAi. (A) Left: Representative images centered on chromosomes of embryos expressing mCherry::H2B (magenta) and either GFP-tagged MEL-28^ELYS, NPP-6^NUP160, NPP-15^NUP133, NPP-25^TMEM33, NPP-12^NUP210, NPP-19^NUP35, or NPP-7^NUP153 (green) during pronuclei migration in the indicated conditions. Scale bar, 5 μm. Right: Quantification of the normalized GFP-tagged MEL-28^ELYS, NPP-6^NUP160, NPP-15^NUP133, NPP-25^TMEM33, NPP-12^NUP210, NPP-19^NUP35, or NPP-7^NUP153 integrated intensity in an ROI centered around the maternal and paternal pronuclei in the indicated conditions. Error bars correspond to the standard error of the mean. Mann–Whitney test (****P < 0.0001).