Nucleoporins with a membrane-binding domain could contribute to interkinetic envelope integrity. (A) Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::LEM-2^LEMD2/3 (green) during interkinesis and interphase in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the normalized GFP::LEM-2^LEMD2/3 integrated intensity over time from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue, npp-6^NUP160(RNAi) in turquoise, npp-25^TMEM33(RNAi) in dark orange, npp-15^NUP133(RNAi) in light orange, npp-12^NUP210(RNAi) in gray, npp-19^NUP53(RNAi) in pink, and npp-7^NUP153(RNAi) in light brown. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann–Whitney test on the mean value of GFP::LEM-2^LEMD2/3 intensity in interkinesis (*P < 0.05, **P < 0.01, ****P < 0.0001). (B) Quantification of the normalized GFP::LEM-2^LEMD2/3 intensity at 300 s after anaphase I onset in the indicated conditions. Mann–Whitney test (***P < 0.001 and ****P < 0.0001).