MEL-28^ELYS is required for the interkinetic envelope integrity. (A) Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (gray) during anaphase I and interkinesis in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. (B) Two-dimensional single sections of two ROIs centered on each chromosomal set of a control (top) and a MEL-28^ELYS–depleted (bottom) oocyte. Scale bars, 1 µm. (C) Three-dimensional reconstructions centered on chromosomes of a control oocyte (top) and a MEL-28^ELYS-depleted oocyte (bottom) viewed from two different angles. Scale bar, 1 µm. Chromosomes in magenta, membranes in contact with chromosomes in green, membranes distant from chromosomes in orange, vesicles in yellow, mitochondria in purple, endoplasmic reticulum in blue, and plasma membrane in gray. Scale bars, 1 µm. (D) Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::LEM-2^LEMD2/3 (green) during meiosis I and II in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. The same control oocyte is displayed in Fig. 1 E and Fig. S2 E. (E) Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::BAF-1^BAF (green) during meiosis I and II in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the GFP::BAF-1^BAF integrated intensity normalized over background from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue, mel-28(RNAi) in purple. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann-Whitney test on the mean value of GFP::BAF-1^BAF intensity in interkinesis.