VRK-1 ^VRK is essential for regulating BAF-1 ^BAF and LEM-2 ^LEMD2/3 recruitment and for the integrity of the interkinetic envelope. (A) Left: Schematics of BAF-1^BAF and LEM-2^LEMD2/3 localization in interphase. Right: At mitotic entry, VRK-1^VRK1 phosphorylates BAF-1^BAF and disrupts its chromatin binding. (B) Representative images of an ROI centered around chromosomes from oocytes expressing GFP::H2B and VRK-1^VRK1::mCherry (n = 12) during interkinesis and interphase. Timings indicated at the bottom left corner of images are from anaphase I onset. Scale bar, 5 μm. (C and D) Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and (C) GFP::BAF-1^BAF or (D) GFP::LEM-2^LEMD2/3 (green) during interkinesis and interphase in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the normalized GFP::LEM-2^LEMD2/3 integrated intensity over time from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue and vrk-1(RNAi) in light brown. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann–Whitney test on the mean value of GFP::LEM-2^LEMD2/3 intensity in interkinesis (***P < 0.001). (E) Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (gray) during anaphase I and interkinesis in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. (F) Left: Three-dimensional reconstructions centered on chromosomes of a control oocyte (top) and a VRK-1^VRK1–depleted oocyte (bottom) viewed from two different angles. Scale bar, 1 µm. Right: Two-dimensional single sections of two ROIs centered on each chromosomal set. Chromosomes in magenta, membranes in contact with chromosomes in green, and plasma membrane in gray. Scale bar, 1 µm. The same control oocyte is displayed in Fig. 5 C.