BAF-1 ^BAF is essential for the integrity of the interkinetic envelope, for INM protein localization, and for normal chromosome segregation. (A) Left: Three-dimensional reconstructions centered on chromosomes of a mid-interkinesis control oocyte (AID::BAF-1^BAF, No auxin) (top) and a mid-interkinesis BAF-1^BAF–depleted oocyte (AID::BAF-1^BAF, 4 mM auxin, baf-1(RNAi)) (bottom) viewed from two different angles. Scale bar, 1 µm. Right: two-dimensional single sections of two ROIs centered on each chromosomal set. Chromosomes in magenta, membranes in contact with chromosomes in green, and plasma membrane in gray. White arrows indicate membrane fragments within the chromosomal sets in the absence of BAF-1^BAF. Scale bar, 1 µm. (B) Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::LEM-2^LEMD2/3 (green) during interkinesis and interphase in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the normalized GFP::LEM-2^LEMD2/3 integrated intensity over time from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue, baf-1(RNAi) in light blue. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann-Whitney test on the mean value of GFP::LEM-2^LEMD2/3 intensity in interkinesis (****P < 0.0001). (C) Representative time-lapse images centered on chromosomes of oocytes expressing GFP::TBA-2^α-tubulin (green) and mCherry::HIS-11^H2B (magenta) during meiosis I in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. (D) Left: Kymographs showing a pair of segregating chromosomes in GFP::TBA-2^α-tubulin (green) and mCherry::HIS-11^H2B (magenta)–expressing oocytes from anaphase I onset in the indicated conditions. Scale bar, 5 µm. Right: Quantification of the distance between the two sets of segregating chromosomes over time from anaphase I onset in control oocytes (dark blue) and BAF-1^BAF–depleted oocytes (light blue). Error bars correspond to the standard error of the mean. Mann–Whitney test on the mean distance between the segregating chromosomal sets during interkinesis in both conditions (**P < 0.01).