BAF-1^BAF is required for the recruitment of LEM-2^LEMD2/3 and EMR-1^Emerin at the chromosome surface in interkinesis. (A) Representative images centered on chromosomes of fixed embryos showing the immunolocalization of LEM-2^LEMD2/3, DNA, Tubulin, and BAF-1^BAF during pronuclei migration in control conditions, baf-1(RNAi), and/or 4 mM auxin treatment. Scale bar, 5 µm. (B) Quantifications of the mean brood size (left) and the percentage of embryonic viability (right) in N2 (control) and JDU647 (AID::BAF-1^BAF) strains. *P < 0.05. (C) Two-dimensional single sections of two ROIs centered on each chromosomal set of a control oocyte (AID::BAF-1^BAF, No auxin) (top) and a BAF-1^BAF–depleted oocyte (AID::BAF-1^BAF, 4 mM auxin, baf-1(RNAi)). Scale bar, 1 µm. (D) Left: Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::EMR-1^Emerin (green) during meiosis I and II in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. Right: Quantification of the GFP::EMR-1^Emerin integrated intensity normalized over a background from anaphase I onset to interphase for the MII chromosomal set. Control in dark blue, baf-1(RNAi) in light blue. Error bars correspond to the standard error of the mean. The orange box indicates interkinesis. Mann–Whitney test on the mean value of GFP::EMR-1^Emerin intensity in interkinesis (****P < 0.0001). (E) Representative time-lapse images centered on chromosomes of oocytes expressing mCherry::H2B (magenta) and GFP::LEM-2^LEMD2/3 (green) during meiosis I and II in the indicated conditions. Timings indicated at the bottom left corners of images are from anaphase I onset. Scale bar, 5 μm. The same control oocyte is displayed in Fig. 1 E and Fig. S3 D. (F and G) Quantifications of the surface of the MII chromosomal set (F) and of tubulin intensity (G) over time from anaphase I onset in control oocytes (dark blue) and BAF-1^BAF–depleted oocytes (light blue). Error bars correspond to the standard error of the mean.