Antibody-mediated hepatic clearance of serotype-39 S. pneumoniae and serotype-K50 K. pneumoniae. (A and B) Bacteremia kinetics (A) and bacterial distribution at 30 min (B) of WT, μMT, C3^−/−, or C3^−/− μMT mice i.v. infected with 10⁶ CFU of TH16827³⁹. n = 6. (C and D) Quantitation of bacteria immobilized on KCs (C) and representative IVM images of liver sinusoids (D) of WT, μMT, C3^−/−, or C3^−/− μMT mice i.v. infected with 5 × 10⁷ CFU of TH16827³⁹. Scale bar, 10 μm. n = 2. The clearance processes are demonstrated in Video 8. (E and F) Bacteremia kinetics (E) and bacterial distribution at 30 min (F) of WT, μMT, C3^−/−, or C3^−/− μMT mice i.v. infected with 10⁶ CFU of TH17033^K50. n = 6. Data were representative results (C and D) or pooled from two independent experiments (A, B, E, and F). Ordinary two-way ANOVA with Tukey’s multiple comparisons test (A and E) and one-way ANOVA with Tukey’s multiple comparisons test (C) were performed. ***, P < 0.001; ****, P < 0.0001; ns, not significant.