Recognition of other capsule types by anti-CPS10A antibodies. (A) ELISA detection of X24-IgM, -IgG3 (left) and X44-IgM, -IgG3 (right) binding to CPS10A. n = 3. (B and C) Bacteremia kinetics of μMT mice i.v. treated with 0, 2.5, and 5 μg X24 or X44-IgM (B) and -IgG3 (C) before i.v. infection with 10⁶ CFU of TH860^10A. n = 6. (D) ELISA detection of antibodies to CPSs with galactose branch in WT mouse serum. n = 6. (E) Bacteremia kinetics of mice i.v. treated with 400 μg CPS39 before infection, as in B. n = 6. (F) The repeating unit structures of CPSs for serotypes 10A and 39 of S. pneumoniae and serotype K50 of K. pneumoniae. (G) ELISA detection of IgG and IgM to K. pneumoniae K50 and K10 capsules in mouse serum. n = 6. (H) Bacteremia kinetics of mice i.v. inoculated with 800 μg CPS of K. pneumoniae K50 (CPSK50) before i.v. infection with 10⁶ CFU of K. pneumoniae K50. n = 6. Data were all pooled from two independent experiments. Ordinary two-way ANOVA with Tukey’s multiple comparisons test (B, C, E, and H) and one-way ANOVA with Tukey’s multiple comparisons test (G) were performed. ***, P < 0.001; ****, P < 0.0001.