Indispensable role of the complement system in nAb -mediated Spn10A capture. (A) Mass spectrometry results showing enriched complement components by CPS10A-beads compared with that of CPS10A∆wcrG-beads. n = 3. The detailed results are listed in Table S1. (B) Bacterial distribution at 5 min of WT, C1qa^−/−, or C3^−/− mice i.v. infected with 10⁶ CFU of TH860^10A. n = 6. (C) ELISA detection of IgG and IgM to CPS10A in serum of complement- and C3-receptor-deficient mice. n = 5. (D) TH860^10A binding to primary mouse KCs with incubation of 10% WT, C1qa^−/−, C3^−/−, or μMT serum. n = 6. (E) Bacterial distribution at 5 min of C3-receptor-deficient mice i.v. infected with 10⁶ CFU of TH860^10A. n = 6. (F) TH860^10A binding to primary mouse KCs from C3-receptor-deficient mice with incubation of 10% WT serum. n = 6. Data are representative results (C) or pooled from two independent experiments (A, B, and D–F). Ordinary one-way ANOVA with Tukey’s multiple comparisons test (D and F) was performed. **, P < 0.01; ****, P < 0.0001.