Figure 4.
Clearance of Spn10A mediated by anti-CPS10A nAbs. (A) Bacteremia kinetics of WT and μMT mice i.v. infected with 106 CFU of TH86010A or TH291214. n = 6. (B–D) Bacteremia kinetics of antibody-deficient mice i.v. treated with various levels of mouse serum (B), purified natural IgG (C), or purified natural IgM (D) before i.v. infection, as in A. n = 6. (E) Bacteremia kinetics of male and female mice infected as in A. n = 6. (F) Representative IVM images of liver sinusoids (left) and quantitation of bacteria immobilized on KCs (right) of antibody-deficient mice i.v. infected with TH86010A or TH291214. Scale bar, 10 μm. n = 2. The clearance processes are demonstrated in Videos 3 and 4. (G) Representative confocal images showing TH86010A or TH291214 binding to primary mouse KCs supplemented with 20% serum of normal or antibody-deficient mice. KC-tethered bacteria are quantified in the right panel. Scale bar, 10 μm. n = 5. (H and I) Survival rate of antibody-deficient mice i.v. (H) or intranasally (i.n.) (I) infected with 107 CFU of TH86010A or TH291214. n = 10. (J) ELISA detection of IgG and IgM to CPS10A in serum of WT and Cd19−/− mice. n = 3. (K) Bacteremia kinetics of WT or Cd19−/− mice i.v. infected with 106 CFU of TH86010A or TH291214. n = 6. (L) Representative IVM images of liver sinusoids (left) and quantitation of bacteria immobilized on KCs (right) of Cd19−/− mice i.v. infected with 5 × 107 CFU of TH86010A or TH291214. Scale bar, 10 μm. n = 2. The processes of bacterial capture are demonstrated in Videos 4 and 5. Data were representative results (F, G, and L) or pooled from two independent experiments (A–E and H–K). Ordinary two-way ANOVA with Tukey’s (A–E and K) or Sidak’s (J) multiple comparisons test, unpaired t test (F, G, and L) and log-rank test (H and I) were performed. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.

Clearance of Spn10A mediated by anti-CPS10A nAb s. (A) Bacteremia kinetics of WT and μMT mice i.v. infected with 106 CFU of TH86010A or TH291214. n = 6. (B–D) Bacteremia kinetics of antibody-deficient mice i.v. treated with various levels of mouse serum (B), purified natural IgG (C), or purified natural IgM (D) before i.v. infection, as in A. n = 6. (E) Bacteremia kinetics of male and female mice infected as in A. n = 6. (F) Representative IVM images of liver sinusoids (left) and quantitation of bacteria immobilized on KCs (right) of antibody-deficient mice i.v. infected with TH86010A or TH291214. Scale bar, 10 μm. n = 2. The clearance processes are demonstrated in Videos 3 and 4. (G) Representative confocal images showing TH86010A or TH291214 binding to primary mouse KCs supplemented with 20% serum of normal or antibody-deficient mice. KC-tethered bacteria are quantified in the right panel. Scale bar, 10 μm. n = 5. (H and I) Survival rate of antibody-deficient mice i.v. (H) or intranasally (i.n.) (I) infected with 107 CFU of TH86010A or TH291214. n = 10. (J) ELISA detection of IgG and IgM to CPS10A in serum of WT and Cd19−/− mice. n = 3. (K) Bacteremia kinetics of WT or Cd19−/− mice i.v. infected with 106 CFU of TH86010A or TH291214. n = 6. (L) Representative IVM images of liver sinusoids (left) and quantitation of bacteria immobilized on KCs (right) of Cd19−/− mice i.v. infected with 5 × 107 CFU of TH86010A or TH291214. Scale bar, 10 μm. n = 2. The processes of bacterial capture are demonstrated in Videos 4 and 5. Data were representative results (F, G, and L) or pooled from two independent experiments (A–E and H–K). Ordinary two-way ANOVA with Tukey’s (A–E and K) or Sidak’s (J) multiple comparisons test, unpaired t test (F, G, and L) and log-rank test (H and I) were performed. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.

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