VEGF-C prophylaxis prevents microglia expansion after ischemic stroke. (A–D) Representative images and quantification of GFAP+ astrocytes (A), NeuN+ neurons (B), PDLX+ blood vessels (C), and Iba1+ immune cells (D) in the ipsilateral (Ipsi) and contralateral (Contra) hemispheres of AAV-CTRL and AAV-VEGF-C mice at 7 d-pso. **P < 0.01, ***P < 0.001, ****P < 0.0001; one-way ANOVA and Bonferroni’s post hoc test. Data are represented as mean ± SEM, n = 5–7 mice in each experimental group. (E–G) Bulk transcriptomic analysis of CD11b+ cells harvested from the brains of mice treated with AAV-mVEGF-C or AAV-CTRL at 7 d-pso or in healthy adults. (E) Volcano plot of DEGs between VEGF-C– and CTRL-treated adult mice. (F) Volcano plot of DEGs between VEGF-C– and CTRL-treated mice at day 7 after stroke. (G) HALLMARK GSEA dot plot showing the most upregulated and downregulated pathways in the AAV-mVEGF-C group compared to AAV-CTRL at 7 d-pso. Scale bar: 35 µm (A–D).
Figure 7.

VEGF-C prophylaxis prevents microglia expansion after ischemic stroke. (A–D) Representative images and quantification of GFAP+ astrocytes (A), NeuN+ neurons (B), PDLX+ blood vessels (C), and Iba1+ immune cells (D) in the ipsilateral (Ipsi) and contralateral (Contra) hemispheres of AAV-CTRL and AAV-VEGF-C mice at 7 d-pso. **P < 0.01, ***P < 0.001, ****P < 0.0001; one-way ANOVA and Bonferroni’s post hoc test. Data are represented as mean ± SEM, n = 5–7 mice in each experimental group. (E–G) Bulk transcriptomic analysis of CD11b+ cells harvested from the brains of mice treated with AAV-mVEGF-C or AAV-CTRL at 7 d-pso or in healthy adults. (E) Volcano plot of DEGs between VEGF-C– and CTRL-treated adult mice. (F) Volcano plot of DEGs between VEGF-C– and CTRL-treated mice at day 7 after stroke. (G) HALLMARK GSEA dot plot showing the most upregulated and downregulated pathways in the AAV-mVEGF-C group compared to AAV-CTRL at 7 d-pso. Scale bar: 35 µm (A–D).

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