Analysis of Vegfc and VEGF-C receptor gene expression. (A) sn-RNAseq analysis. Dot plot representation of transcript expression among the different clusters using Log normalized and zero centered expression in each cluster (AAV-VEGF-C, AAV-CTRL). (B–K) Immunophenotyping of Vegfr3-expressing cells in the brain of Vegfr3::YFP reporter mice. (B) Pial and brain penetrating blood vessels (arrowheads) as well as subsets of neural cells express YFP in the cortex. (C) Colocalization of YFP and PDLX/CD31 in pial vessels (white arrowhead). (D) YFP labeling along large veins expressing Von Willebrand factor (vWF) and exiting the cortex. (E and F) Most small cerebral vessels lack YFP expression (E), although YFP can be detected at branching points of VEGFR2⁺ capillaries (F). (G–K) Other types of brain parenchymal cells express YFP, including astrocytes and their end-feet (GFAP⁺; white arrowheads in G and H) and subsets of neurons (NeuN⁺; white arrowheads in I). Microglial cells do not express YFP (J), in contrast with VEGFR-2⁺ choroid plexus blood vessels (K).