snRNA-seq reveals VEGF-C induced pathways in forebrain cells. (A) Overview of the experimental procedure. (B) TSNE plots showing cell-type-specific cluster annotation. (C) WikiPathways analysis of signaling pathway alterations induced by VEGF-C among brain cell clusters. Oligodendrocytes, astrocytes, endothelial cells, cortical pyramidal (cx pyram) neurons, inhibitory neurons, Sv2c interneurons, and mix neurons show >180 DEGs (Padj < 0.05 and abs(log₂FC) > 0.58), with pathways of at least 20 genes and adjusted P values <0.05 in at least one cluster. (D–F) Functionally organized network from ClueGO analysis visualized with Cytoscape in inhibitory neurons (D), astrocytes (E), and endothelial cells (F). Only main pathways are represented. Main terms are represented with color. Dot size represents the number of DEGs in common between pathways. Vip, vasoactive intestinal polypeptide; Hyp, hypothalamus; OPC, oligodendrocyte precursor cell.